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3.2K Views. Cincinnati Children’s Hospital Medical Center. The most important advantages of this technique are reversibility, reduction in cost of the animals and maintenance, and a shorter pregnancy period. Moreover, animal mortality is reduced because there is no need for hysterectomy. And there are wider opportunities for genetic workup.This technique can be used for investigating the pathophysiology of the congenital diaphragmatic hernia. It can also be applied to understanding other diseases in the lung, such as cows and pulmonary hypopla...